Comparison of susceptibility patterns using commercially available susceptibility testing methods performed on prevalent Candida spp.
نویسندگان
چکیده
منابع مشابه
Comparison of two microdilution methods for testing susceptibility of Candida spp. to voriconazole.
The growing number of fungal infections, coupled with emerging resistance to classical antifungal agents, has led to the development of new agents, among them voriconazole. Susceptibility to voriconazole was tested by using two microdilution techniques: the reference method described in National Committee for Clinical Laboratory Standards document M27-A2 and a colorimetric method, Sensititre Ye...
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In general, methods to test the susceptibility of fungi to antifungal drugs require standardized techniques, but so far there is no methodology that is widely applicable to dermatophytes. Here we introduced modifications to the protocols from documents of the National Committee for Clinical Laboratory Standards (CLSI) M38-A and the Antifungal Susceptibility Testing Subcommittee of the European ...
متن کاملFluconazole susceptibility testing of Candida inconspicua clinical isolates: comparison of four methods.
Sir, During the past 4 years, isolation of Candida inconspicua from clinical samples has increased in our laboratory. Fluconazole MIC values for C. inconspicua isolates are relatively high, as determined by standard broth microdilution (BMD), Etest or Fungitest, – 4 and according to the standard BMD method they fall mainly into the susceptible-dose dependent (S-DD) or resistant (R) categories, ...
متن کاملRapid Susceptibility Testing and Microcolony Analysis of Candida spp. Cultured and Imaged on Porous Aluminum Oxide
BACKGROUND Acquired resistance to antifungal agents now supports the introduction of susceptibility testing for species-drug combinations for which this was previously thought unnecessary. For pathogenic yeasts, conventional phenotypic testing needs at least 24 h. Culture on a porous aluminum oxide (PAO) support combined with microscopy offers a route to more rapid results. METHODS Microcolon...
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Optimal methods for susceptibility testing of Candida spp. with flucytosine have not been determined. Breakpoints were recommended in 1984, but never validated. In this study, we compared the 1984 recommended macrodilution broth method (using an 80% endpoint) with a modification of the more recent NCCLS-recommended microdilution broth method with three endpoints-spectrophotometric 50% and 80% a...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2016
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.000383